Differential stability of human interleukin 1 beta fragments expressed in yeast
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection
- Vol. 1 (5) , 433-437
- https://doi.org/10.1093/protein/1.5.433
Abstract
We have made deletions in a human interleukin 1 beta cDNA cloned in a yeast vector capable of directing the expression and secretion of foreign protein. Deletions at either the 5′ or 3′ end of the cDNA result in a dramatic drop in yield of protein. An investigation of some possible causes points to in stability of the truncated peptides as a major factor contributing to low yield. The results suggest that correct folding of mature interleukln 1 into a protease resistant form requires almost the entire polypeptide.This publication has 11 references indexed in Scilit:
- A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1 beta in Saccharomyces cerevisiae.The EMBO Journal, 1987
- Structure-function analysis of murine interleukin 1: biologically active polypeptides are at least 127 amino acids long and are derived from the carboxyl terminus of a 270-amino acid precursor.Proceedings of the National Academy of Sciences, 1986
- Binding and internalization of interleukin 1 by T cells. Direct evidence for high- and low-affinity classes of interleukin 1 receptor.The Journal of Experimental Medicine, 1986
- Expression of the amino terminal part of synthetic human growth hormone gene and somatomedin-like activity of expressed proteinProtein Engineering, Design and Selection, 1986
- Detection and characterization of high affinity plasma membrane receptors for human interleukin 1.The Journal of Experimental Medicine, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Ovomucoid intervening sequences specify functional domains and generate protein polymorphismCell, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Assay of proteins in the presence of interfering materialsAnalytical Biochemistry, 1976