Principles of Competitive Binding Assays (Saturation Analyses). II. Sequential Saturation

1 January 1974

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 20 (1) , 5-14
https://doi.org/10.1093/clinchem/20.1.5

Abstract

The principles of sequential saturation as a form of competitive binding assays are discussed in detail and differentiated from those of equilibrium techniques. The rationale of its applicability in practice and its potential to increase sensitivity under certain assay conditions are demonstrated with three binding systems involving digoxin, insulin, and folates. The advantages and disadvantages of the sequential saturation technique are outlined.

Keywords

RADIOIMMUNOASSAY
EQUILIBRIUM ASSAY TECHNIQUE COMPARED WITH SEQUENTIAL SATURATION TECHNIQUE
DOSE-RESPONSE CURVES
DIGOXIN-
INSULIN-
AND FOLATE-BINDING SYSTEMS
ADVANTAGES AND DISADVANTAGES OT THE SEQUENTIAL SATURATION TECHNIQUE