Localisation of the MRC OX‐2 Glycoprotein on the Surfaces of Neurones

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Abstract
The MRC OX-2 monoclonal antibody recognizes membrane glycoproteins of MW 41,000 (daltons) in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was present throughout the cerebellum with staining of both gray and white matter. Blood vessels were also stained. The Purkinje cell layer appeared to be unlabeled. Double-immunofluorescence staining of cerebellar interneuron cultures with MRC OX-2 antibody and tetanus toxin showed that all tetanus-positive cells (neurons) were MRC OX-2-positive. Glial fibrillary acidic protein-positive astrocytes were not labeled by MRC OX-2 antibody. Thus, OX-2 antigen is one of the few biochemically characterized components of neuronal membranes and its properties are compared with those of the neuronal membrane glycoprotein Thy-1.