Transient Stirred‐Tank Reactors Operating with Immobilized Enzyme Systems: Analysis and Simulation Models and Their Experimental Checking
- 1 March 1993
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 9 (2) , 166-173
- https://doi.org/10.1021/bp00020a008
Abstract
A mathematical model applicable to the analysis, design, and simulation of heterogeneous enzymatic processes reacting in a stirred-tank reactor has been formulated. The model develops the unsteady-state design equations for such reactors and also the diffusion reaction equation in the spherical catalyst particles for reversible Michaelis-Menten kinetics. This structured general model provides an overview of almost all of the processes occurring in an immobilized enzyme reactor system. Although the analysis presented here is limited to certain situations, it can be extended to other support geometries, enzyme kinetics, and reactor configurations. To check the model, the experimental data obtained with immobilized beta-galactosidase were compared to those theoretically predicted.Keywords
This publication has 12 references indexed in Scilit:
- A comparison of different methods of β-galactosidase immobilizationProcess Biochemistry, 1991
- Evaluation of the effectiveness factor along immobilized enzyme fixed-bed reactors: Design of a reactor with naringinase covalently immobilized into glycophase-coated porous glassBiotechnology & Bioengineering, 1987
- Analysis of diffusion effects on immobilized enzymes on porous supports with reversible Michaelis-Menten kineticsEnzyme and Microbial Technology, 1986
- Modeling enzyme immobilization in porous solid supportsBiotechnology & Bioengineering, 1982
- Effect of diffusion limitations and floc size distributions on fermentor performance and the interpretation of experimental dataBiotechnology & Bioengineering, 1979
- SIMULATION OF AN IMMOBILIZED ENZYME PARTICLE USING β‐GALACTOSIDASE ADSORBED TO A PHENOL‐FORMALDEHYDE RESINJournal of Food Process Engineering, 1977
- Mass transfer and biochemical reaction in enzyme membrane reactor systems—II: Expanded analysis for single enzyme systems: effects of enzyme intermediates, denaturation and elutionChemical Engineering Science, 1975
- Water insoluble enzyme columns: Kinetic study on steady state and transient conditionsBiotechnology & Bioengineering, 1974
- Effect of internal diffusion in heterogeneous enzyme systems: Evaluation of true kinetic parameters and substrate diffusivityJournal of Theoretical Biology, 1973
- Determination of protein: A modification of the lowry method that gives a linear photometric responseAnalytical Biochemistry, 1972