A Liquid Radiochromatographic Method for Following the Synthesis and Hydrolysis of a Doubly (3H,14C) Radiolabeled Alkyl Betainate

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Abstract
In order to study the rate of hydrolysis of long-chain alkyl betainates in various biological environments, a technique was developed based on the introduction of different radioisotopes in the betaine and alkyl groups, respectively. The hydrolytic reaction process was then followed by liquid reversed-phase chromatography with on-line radioactivity monitoring of the reactant and products using discrete counting windows for the two radioisotopes. The 3H/14C-ratio was used to evaluate possible losses due to adsorption to the biological material. A method for the synthesis of alkyl betainates, having 3H in the alkyl chain and 14C in the betaine part, is also described. Radiochromatography was used to follow the synthesis.