Effect of adding cysteine to brain-heart infusion broth on the isolation of Bacteroides fragilis from experimental blood cultures.

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Abstract
Adding 0.05% cysteine to brain-heart infusion broth greatly improved the recovery of 3 strains of B. fragilis from simulated blood cultures (horse blood). No Bacteroides were isolated after 24 h incubation in brain-heart infusion broth without added cysteine; this was therefore a poor medium for the isolation of B. fragilis. Results with brain-heart infusion broth containing 0.05% cysteine were similar to those obtained with commercially prepared Thiol and Brewer''s thioglycollate media.