Repair of dGMP hydroxyl radical adducts by verbascoside via electron transfer: a pulse radiolysis study
- 1 January 1996
- journal article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 69 (4) , 481-485
- https://doi.org/10.1080/095530096145779
Abstract
The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it was found that the transient absorption spectrum of the hydroxyl adduct of dGMP decays with the formation of that of the phenoxyl radical of VER, several tens of microseconds after the electron pulse. From the formation kinetics of the phenoxyl radical of VER, the rate constant of the repair reaction was determined to be 1.12 x 10(9) dm(3) mol(-1) s(-1).Keywords
This publication has 12 references indexed in Scilit:
- Verbascoside Isolated from Lantana camara, an Inhibitor of Protein Kinase CJournal of Natural Products, 1991
- Endogenous mutagens and the causes of aging and cancerMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1991
- Phenolic compounds and an analog as superoxide anion scavengers and anti oxidantsBiochemical Pharmacology, 1991
- Phenylpropanoid and iridoid glycosides fromPedicularis spicataPhytochemistry, 1991
- Modification of DNA bases in mammalian chromatin by radiation-generated free radicalsBiochemistry, 1990
- Pharmacological Effects of Three Phenylpropanoid Glycosides fromMussatiaPlanta Medica, 1990
- Purine bases, nucleosides, and nucleotides: aqueous solution redox chemistry and transformation reactions of their radical cations and e- and OH adductsChemical Reviews, 1989
- Triterpene glycosides from Mussatia speciesPhytochemistry, 1989
- Critical Review of rate constants for reactions of hydrated electrons, hydrogen atoms and hydroxyl radicals (⋅OH/⋅O− in Aqueous SolutionJournal of Physical and Chemical Reference Data, 1988
- Somatic mutation, DNA damage and cytotoxicity induced by benzo[a]pyrenedione/benzo[a]pyrenediol redox couples in cultured mammalian cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1986