Fractionation of cell constituents of Bacillus megaterium in a polymer two‐phase system

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Abstract
The distribution of various cell constituents of Bacillus megaterium has been studied in an aqueous two‐phase system containing the two polymers dextran and polythylene glycol. The difference in behaviour between cell walls, lipid granules and soluble cell material are such that a considerable fractionation of a suspension of disrupted cells may be obtained after a few counter‐current distributions. A mixture of whole cells and protoplasts can also be fractionated in this phase system. If sodium chloride is added to the phase system, vegetative cells and spores can be separated from each other.