Effects of Metal-Binding Fractions of Tobacco Smoke on in Vitro Activity of Enzymes

Publisher Website
Google Scholar
Abstract
To Investigate the role of metal-binding agents present In the environment on trace metal metabolism, we have successfully isolated from the numerous constituents of tobacco smoke condensate (TSC), two fractions: protonated ligands (II-F2) and nonprotonated ligands (II-F3). Both these fractions, along with a copper containing fraction (I-F2), have been tested for inhibitory activity toward catalase, galactose oxidase and glucose oxidase. Galactose oxidase and catalase are metalloenzymes and glucose oxidase is a glycoprotein containing flavin-adenine dinucleotide and no metals. Both fractions, II-F2 and II-F3, strongly inhibited galactose oxidase, a coppercontaining enzyme, while they did not affect glucose oxidase. The protonated ligands (II-F2) and their copper complexes (I-F2) inhibited catalase but the nonprotonated ligands (II-F3) had no effect on this enzyme.