Vertebrates exhibit evolutionarily conserved asymmetries in the pattern of internal organ placement that are essential for their normal physiological function. Left‐right asymmetries in organ situs are dependent upon the formation of an intact left‐right axis during embryogenesis. Recently many of the molecular components involved in the initiation and maintenance of the left‐right axis have been described. These molecules and their function in promoting left‐right asymmetries are reviewed.