Lipoprotein lipase in adipose tissue and plasma triglceride clearance in patients with primar hpertriglceridaemia

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Abstract
The activity of lipoprotein lipase (E.C. 3.1.1.3) extracted and released from adipose tissue was measured in obese subjects with mild or severe hypertriglyceridemia. The Intralipid tolerance test was studied as a measure of in vivo clearance of exogenous triglyceride from plasma. Plasma triglyceride clearance was reduced in patients with severe hypertriglyceridemia (> 4 mmol/l); this was associated with reduced activities of extracted lipoprotein lipase in adipose tissue compared to patients with mild hypertriglyceridemia (2-4 mmol/l). A course of insulin therapy (20 u/day) for 1 wk increased the extracted, but not the released component of the enzyme from adipose tissue of hypertriglyceridemic patients. Serum (25% v/v) potentiated the release of lipoprotein lipase from adipose tissue of normo- but not hypertriglyceridemic patients. The effect of serum on enzyme release was abolished if lipoprotein-deficient serum was used. Addition of very low density lipoprotein (VLDL) or high density liproprotein (HDL) restored the ability of delipidated serum to augment release of enzyme from adipose tissue, but this was not observed with low density lipoprotein (LDL). A defect in the release of lipoprotein lipase activity in adipose tissue may contribute to the development of hypertriglyceridemia in this group of patients.