Determination of Glucose Utilization in the Dog with [2-3H], [6-3H]-, and [U-14C]Glucose

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Abstract
Parameters of in vivo glucose metabolism in adult female dogs fasted for 48 hr were estimated after a single injection of [2-3H]- or [6-3H]glucose in combination with [U-14C]glucose. The glucose replacement rate estimated with [2-3H]glucose averaged 4.6 mg/min/kg and was about 25% higher than the values obtained with [6-3H]glucose. Estimates of glucose-carbon recycling were more than twice as high (31 vs 14%) when [2-3H]glucose was utilized as tracer than when [6-3H]glucose was utilized. These differences suggest the presence of a “futile” cycle between glucose and glucose-6-phosphate in the dog.