Metabolism of platelet‐activating factor by blood platelets and plasma
- 1 August 1983
- Vol. 18 (8) , 534-538
- https://doi.org/10.1007/bf02535393
Abstract
High performance liquid chromatography in combination with a radioactivity detector was used to study the metabolism of platelet-activating factor (1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by washed platelets, platelet-free plasma and platelet-rich plasma obtained from rabbits and humans. Degradation of platelet-activating factor in plasma was completely inhibited by diisopropylfluorophosphate and was partially inhibited by ethylenediamine tetraacetic acid. Washed platelets metabolized platelet-activating factor not only to the 2-lyso compound but also, by reacylation of this lyso intermediate, to an analogue of platelet-activating factor probably containing a long-chain acyl group at thesn-2 position. These transformations occurred, but to a lesser extent, in platelet-rich plasma.Keywords
This publication has 26 references indexed in Scilit:
- Ether phospholipids in guinea pig polymorphonuclear leukocytes and macrophages Occurrence of high levels of 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Activities of enzymes that metabolize platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) in neutrophils and eosinophils from humans and the effect of a calcium ionophoreBiochemical and Biophysical Research Communications, 1982
- In vivo metabolism of a new class of biologically active phospholipids: 1-alkyl-2-acetyl--glycero-3-phosphocholine, a platelet activating-hypotensive phospholipidLife Sciences, 1981
- Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor and a hypotensive lipid) by cholinephosphotransferase in various rat tissuesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Preliminary studies of an acid-labile factor (ALF) in human sera that inactivates platelet-activating factor (PAF)Clinical Immunology and Immunopathology, 1980
- Antihypertensive activity of an alkyl ether analog of phosphatidylcholineBiochemical and Biophysical Research Communications, 1979
- Structural analysis of purified platelet-activating factor by lipasesNature, 1977
- Cyclic adenosine 3',5'-monophosphate inhibits the availability of arachidonate to prostaglandin synthetase in human platelet suspensions.Journal of Clinical Investigation, 1977
- Metabolism of [14C]arachidonic acid by human plateletsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- LEUKOCYTE-DEPENDENT HISTAMINE RELEASE FROM RABBIT PLATELETSThe Journal of Experimental Medicine, 1972