IMMEDIATE-TYPE HYPERSENSITIVITY TO KEYHOLE LIMPET HEMOCYANIN IN DOG

  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (1) , 95-101
Abstract
Long-lasting ITH [immediate type hypersensitivity] was induced [using keyhole limpet hemocyanin (KLH)] in 15 of 16 dogs by the i.v., intradermal (i.d.), inhalation and i.d. plus inhalation routes. This ITH was demonstrated by direct skin reactivity, acute respiratory reactions and peripheral blood leukocyte and respiratory cell histamine release. The reaginic antibodies considered to mediate these reactions were larger than canine IgG [immunoglobulin G], heat labile, 2-mercapto-ethanol sensitive and are probably canine IgE. These reagins also mediate systemic reactions. An unexpected finding was the occurrence of ITH to KLH in 3 dogs which had had no prior experimental exposure to KLH. This ITH was of sufficient intensity to be associated with acute airway reactions to the inhaled antigen.