MALDI Mass Spectrometry for Direct Tissue Analysis: A New Tool for Biomarker Discovery
- 6 July 2005
- journal article
- review article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 4 (4) , 1138-1142
- https://doi.org/10.1021/pr050095+
Abstract
The direct analysis of tissue sections by MALDI mass spectrometry holds tremendous potential for biomarker discovery. This technology routinely allows many hundreds of proteins to be detected over a mass range of ∼2000−70 000 Da while maintaining the spatial localization of the proteins detected. This technology has been applied to a wide range of tissue samples, including human glioma tissue and human lung tumor tissue. In many cases, biostatistical analyses of the resulting protein profiles revealed patterns that correlated with disease state and/or clinical endpoints. This work serves as a review of recent applications and summarizes the current state of technology. Keywords: MALDI MS • imaging • tissues • comparative proteomicsKeywords
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