Production, isolation and characterization of human profilin fromSaccharomyces cerevisiae
- 1 April 1991
- journal article
- Published by Springer Nature in Journal of Muscle Research and Cell Motility
- Vol. 12 (2) , 201-207
- https://doi.org/10.1007/bf01774039
Abstract
The yeastSaccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.Keywords
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