A Rapid Method for Determining T Lymphocyte Levels Using Acid Alpha-Naphthyl Acetate Esterase
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 56 (3) , 189-193
- https://doi.org/10.3109/10520298109067309
Abstract
A method for rapidly determining levels of T lymphocytes in humans is presented. The method involves staining lymphocytes in peripheral blood or buffy coat smears for acid alpha-naphthylacetate esterase for 1 hr at 37 C. After counterstaining with Wright's stain, the percentage of esterase positive cells is quantitated microscopically. The entire procedure takes less than 2 hours. Data are presented which show that the percentage of esterase-positive lymphocytes accurately reflects the percentage of lymphocytes forming E rosettes with sheep erythrocytes.This publication has 5 references indexed in Scilit:
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- Acid Esterase in Human Lymphoid Cells and Leukaemic Blasts: a Marker for T LymphocytesBritish Journal of Haematology, 1977
- Nonspecific acid esterase activity: A criterion for differentation of T and B lymphocytes in mouse lymph nodesEuropean Journal of Immunology, 1975