Formation of Adventitious Embryos in Callus Cultures of ‘Seyval’, a French Hybrid Grape1
Open Access
- 1 May 1977
- journal article
- Published by American Society for Horticultural Science in Journal of the American Society for Horticultural Science
- Vol. 102 (3) , 360-363
- https://doi.org/10.21273/jashs.102.3.360
Abstract
Callus tissue of ‘Seyval’ (Seyve-Villard 5-276) (Vitis sp.), a French hybrid grape, formed adventitious embryos when transferred from a medium with 2,4-dichlorophenoxyacetic acid (2,4-D) to a medium containing 1-napthaleneacetic acid (NAA). Embryos began to turn green and develop into apparently normal vines when placed on a medium free of hormones and vitamins in the light. Histological evidence indicated that plants derived from callus originated from embryo-like structures and not from plantlets or excised buds. Secondary embryoids formed on primary embryoids, and tertiary embryoids occasionally formed on secondary embryoids. More than 50 vines and several hundred adventitious embryoids were obtained from approximately one cubic centimeter of callus.Keywords
This publication has 0 references indexed in Scilit: