DNA Biosensor Investigations in Fish Bile for Use as a Biomonitoring Tool
- 8 January 2003
- journal article
- Published by Taylor & Francis in Analytical Letters
- Vol. 36 (9) , 1887-1901
- https://doi.org/10.1081/al-120023620
Abstract
Polycyclic aromatic hydrocarbons (PAH) are responsible for a number of adverse effects on aquatic biota due to their persistence and potential for accumulation. The recognition and quantification of biliary PAH metabolites from exposed fish represents a valuable tool in the evaluation of warning signals indicative of exposure. In this work, an electrochemical DNA biosensor is proposed as a screening device for the rapid detection of PAH metabolites in fish bile samples. Preliminary studies were performed using the 3-hydroxy-benzo(a)pyrene as a model compound. The effect of the 3-hydroxy-benzo(a)pyrene on the surface-confined DNA was found to be linearly related to the concentration of the PAH derivative in solution. Then, the DNA biosensor was used to investigate the presence of PAH metabolites in fish bile samples. The sensor was able to clearly distinguish PAH-exposed fish bile samples from unexposed control samples. A total analysis time of 7 min was established (including electrode surface pr...Keywords
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