A New Method for Separation of Gibberellins and Growth Inhibitors in Plant Extracts using Sephadex Columns

Open Access
Publisher Website
Google Scholar
Abstract
A method is described for estimating gibberellin levels in plant material, using a separation method avoiding organic solvents. High molecular weight inhibitors were separated from growth-promoting fractions by column chromatography on Sephadex. Of the several grades of Sephadex tried, G15 was found to give the sharpest separation. Ammonium chloride was included in the sample applied to the column to increase the retention of anionic substances. The inhibitory effect of other substances, probably abscisic acid, which are eluted from the gel together with the gibberellins, was reversed by addition of kinetin to test solutions in the lettuce hypocotyl bioassay. Using this method an increase in gibberellin level in cotyledons of Phaseolus multiflorus during germination was measured in terms of GA3 activity, with reference to the lettuce hypocotyl bioassay.

This publication has 0 references indexed in Scilit: