Bioassay System for Determining Ribavirin Levels in Human Serum and Urine

Publisher Website
Google Scholar
Abstract
A simple, sensitive, micromethod was developed to determine levels of ribavirin, or its active metabolic products, in human serum or urine. The procedure utilized the inhibition of measles virus cytopathic effect in [African green monkey kidney] BS-C-1 cells. Based upon maximum dilutions of human serum or urine containing ribavirin which inhibited the measles virus, the bioassay detected ribavirin in concentrations as low as 0.006 .mu.g/ml in serum or 0.03 .mu.g/ml in urine. Herpesvirus 1, parainfluenza virus 3 and reovirus 1 were also tested for sensitivity to ribavirin. Minimum inhibitory concentrations of ribavirin in serum or urine against these other viruses were no lower than 0.32 .mu.g/ml.

This publication has 1 reference indexed in Scilit: