A Simple Multichannel Perifusion Apparatus of Rat Adenohypophysis and Growth Hormone and Prolactin Responses to Partially Purified Bovine Hypothalamic Extracts

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Abstract
A simple multichannel perifusion apparatus for rat adenohypophysis was developed using 2.5 ml disposable syringes as incubation chambers. After placing 1 or 2 pituitaries in each chamber, the pituitaries were perifused at a rate of 50-75 .mu.l/min with KRBG [Krebs-Ringer-bicarbonate-glucose buffer] which was gassed with a 95% O2-5% CO2 mixture. The BHE [bovine hypothalamic extract] fractions used were collected following Sephadex G-25 gel filtration of BHE. When the pituitaries were perifused for intervals of 20 or 40 min with the BHE fractions, the peak responses of GH [growth hormone] and PRL [prolactin] were always achieved within 20 min after initiation of perifusion. The peak values and the amounts of GH and PRL stimulated by BHE were dose related. Both parameters of GH and PRL decreased with repeated pulses of the same dose of BHE.

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