5α-Dihydrotestosterone Binding Protein in Rat Ventral Prostate; Purification, Nuclear Incorporation, and Subnuclear Localization
- 1 January 1975
- journal article
- Published by Japan Endocrine Society in Endocrinologia Japonica
- Vol. 22 (5) , 433-437
- https://doi.org/10.1507/endocrj1954.22.433
Abstract
Treatment of cytosol from the rat ventral prostate with cold acetone (-20 degrees C) evoked a 8 approximately 10-fold increase in the binding capacity with 5alpha-dihydrotestosterone (DHT). Starting from the extract of acetone-dried prostate cytosol, some 400 approximately 600-fold purification of the DHT-binding protein complex was acieved by (NH4)2504 fractionation, DEAE-cellulose chromatography and gel- filtration with Sephadex G-200. The purified 3H-DHT-binding protein complex was incorporated into the nuclei from the ventral prostate in a temperature dependent manner. The similar incorporation was also observed in nuclei from the liver and the kidney...Keywords
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