The Induction of Cellular DNA Synthesis in G1-arrested BHK21 Cells Infected with Adenovirus Type 2

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Abstract
G1-arrested BHK21 [baby hamster kidney] cells infected with adenovirus type 2 were studied to determine the effect of infection on host DNA synthesis in a productive cycle of infection. At various intervals following infection, analysis of the intracellular DNA was carried out by zonal sedimentation centrifugation through alkaline sucrose gradients. Material which sedimented to the position of the virus marker (34S) and a class of high MW DNA (40-100S) were synthesized beginning about 13 h p.i. [post-infection] and continuing up to about 35 h p.i. DNA-DNA hybridization studies on this newly synthesized DNA showed it to be cellular DNA. This material was density labeled with 5-bromodeoxyuridine and centrifuged to equilibrium through alkaline CsCl gradients; it was the product of semi-conservative replication and not of repair synthesis.

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