METHOD FOR ISOLATION OF MATING-LESS MUTANTS FROM RHODOSPORIDIUM TORULOIDES M 1057, A STRAIN OF MATING TYPE a

Abstract
A cell suspension was irradiated with UV for 3 min and a segregation culture performed on a yeast-sucrose agar (YS) for 15 h. Segregates were plated on YS agar and incubated for 5 days. Colonies were replicated on minimal agar medium and colonies not giving brown hyphal colonies were isolated as mating-less mutants. Strains (97) were isolated and grouped by mating tube-forming ability and mating ability.

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