Organization of the human genes for insulin‐like growth factors I and II
Open Access
- 20 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 195 (1-2) , 179-184
- https://doi.org/10.1016/0014-5793(86)80156-9
Abstract
Recently, we have reported the isolation of cDNAs encoding the precursors of insulin‐like growth factors I and II (IGF‐I and II) [(1983) Nature 306, 609‐611; (1985) FEBS Lett. 179, 243‐246. These cDNAs were employed as specific probes to detect and isolate the corresponding genes from human cosmid DNA libraries. Three cosmids were detected, together containing the entire cDNA sequence of IGF‐I, and one cosmid containing the sequence of IGF‐II cDNA. Southern blot hybridization, physical mapping and nucleotide sequence analysis of these cosmids revealed that the IGF‐I and ‐II genes have a discontinous structure. The IGF‐I gene contains at least four exons spanning a region of probably more that 45 kilobasepairs (kb), while the IGF‐II gene consists of at least five exons, spanning a region of 16 kb.Keywords
This publication has 21 references indexed in Scilit:
- Nucleotide sequences of cDNAs encoding precursors of human insulin‐like growth factor II (IGF‐II) and an IGF‐II variantFEBS Letters, 1985
- Localization of insulin-like growth factor genes to human chromosomes 11 and 12Nature, 1984
- Human chromosomal mapping of genes for insulin-like growth factors I and II and epidermal growth factorNature, 1984
- Sequence of a cDNA clone encoding human preproinsulin-like growth factor IINature, 1984
- Insulin-like growth factor II precursor gene organization in relation to insulin gene familyNature, 1984
- Sequence of cDNA encoding human insulin-like growth factor I precursorNature, 1983
- Double cos site vectors: simplified cosmid cloningGene, 1983
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- Primary structure of human insulin‐like growth factor IIFEBS Letters, 1978
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977