Identification and Physiological Characterization of a New Amylolytic Hydrogen Bacterium,Pseudomonas hydrogenovora

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Abstract
Strain 9–5, which was newly isolated from soil as a hydrogen bacterium, was found to be a new species of amylolytic pseudomonad and named Pseudomonas hydrogenovora. The (G+C) content of the DNA of P. hydrogenovora was 62.3–62.5 mole%. Genus Pseudomonas is divided into four sections in “Bergey’s Manual.” P. hydrogenovora showed characteristics intermediate between section II and section III types. Similarities were present in the properties of Strain 9–5 and Pseudomonas saccharophila, an amylolytic hydrogen-oxidizing pseudomonad, but detailed studies revealed many important differences between these two strains. The distribution of hydrogenase in this strain was also examined and found to differ from that in P. saccharophila. P. hydrogenovora showed membrane-bound hydrogenase and very weak, almost negligible soluble hydrogenase activity to reduce NAD.