LYMPHOCYTE CHEMOTAXIS IN INFLAMMATION .6. LYT PHENOTYPE ANALYSIS OF EFFECTOR-CELLS RESPONSIBLE FOR PRODUCING MURINE LYMPHOCYTE CHEMOTACTIC FACTOR

  • 1 January 1983
    • journal article
    • research article
    • Vol. 49  (1) , 95-102
Abstract
Murine lymphocyte chemotactic factor (LCF) was demonstrated in various culture fluids of C3H/HeN lymphoid cells stimulated with specific soluble protein antigen, mitogen or alloantigenic cells. Further experiments, using monoclonal anti-Thy 1.2, anti-Lyt 1.1 and anti-Lyt 2.1 antibodies for negative selection with complement (C), were carried out to characterize the effector-cell populations responsible for producing LCF after these stimuli. Treatment of sensitized lymph node (LN) cells with either anti-Thy 1.2, or anti-Lyt 1.1 and C resulted in an almost complete elimination of the capacity to produce LCF after dinitrophenylated-ovalbumin-stimulation. Spleen cells treated with these antibodies and C before stimulation with either alloantigen (irradiated C576BL/6 spleen cells or concanavalin A [Con A]) yielded almost the same results as those for LN cells. Depletion of Lyt cells, under conditions which fully abrogated the generation of cytotoxic T cells in primary mixed-lymphocyte culture (MLC) and the cytotoxic activity of the cells generated in MLC, had little or no ability to eliminate LCF production in either system. Lyt 1+2- T-cell subpopulations apparently were primarily responsible for LCF production after stimulation with either specific protein antigen, alloantigen or Con A.

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