Characterization of Rice Stripe Virus: a Heavy Component Carrying Infectivity
- 1 August 1982
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 61 (2) , 187-195
- https://doi.org/10.1099/0022-1317-61-2-187
Abstract
Rice stripe virus (RSV) preparations contain a previously unreported nucleoprotein component (nB) which sediments faster in sucrose gradients than middle (M) and bottom (B) components; the latter 2 components are 20-fold more concentrated than nB. EM of purified nB preparations revealed filamentous particles (8 nm wide) and other structures which might be derived from these particles. The nB preparations contained 4 RNA species of MW 0.9 .times. 106, 1.0 .times. 106, 1.4 .times. 106 and 1.9 .times. 106 (2700, 3000, 4100 and 5600 nucleotides, respectively); the 3 smaller RNA may be derived from M and B components contaminating nB. A single protein (MW 3.2 .times. 104) was present in M, B and nB preparations. The nB component appears to be required for infectivity because the planthopper vector (Laodelphax striatellus) became viruliferous after injection with nB but not after injection with M or B.This publication has 0 references indexed in Scilit: