Solubilized DNA-dependent RNA polymerase activities in resting and growing fibroblast
- 22 February 1977
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 16 (4) , 793-797
- https://doi.org/10.1021/bi00623a035
Abstract
The activities of RNA polymerases I and II were measured in 3T6 [mouse fibroblasts] during the transition from the resting to growing state by solubilization of the enzymes followed by chromatography on DEAE-Sephadex columns. The activity of RNA polymerase II remains unchanged during the first 12 h after serum stimulation while the activity of RNA polymerase I increases and closely parallels the increased activity seen in isolated nuclei. Compared to enzyme from resting cells, RNA polymerase I from serum stimulated cells elutes at a lower ammonium sulfate concentration on DEAE-Sephadex chromatography and its activity shows distinctly different dependencies on the concentration of ammonium sulfate and Mg2+. These observations are discussed in relation to the possible mechanism by which 3T6 regulates the synthesis of preribosomal RNA.This publication has 3 references indexed in Scilit:
- Quantitation of elongating form A and B RNA polymerases in chick oviduct nuclei and effects of estradiolCell, 1976
- The quantitation of urinary and spinal fluid proteins by a modified biuret methodClinical Biochemistry, 1976
- Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cellsJournal of Molecular Biology, 1968