Assessment of Centrifugal Partition Chromatography for Determination of Octanol-Water Partition Coefficients

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Abstract
Centrifugal partition chromatography (CPC) has been assessed as a convenient automated method for the determination of octanol-water partition coefficients (Kow) over the range of -0.5 to 2.5 log units. The stationary (Vs) and mobile phase (Vm) volumes, which are needed for the calculation of Kow, are determined in situ by injecting four compounds with known Kow. Vs and Vm were also determined by independent analytical means to demonstrate that this is a direct measurement of Kow from fundamental chromatographic principles with no unexplained fitted parameters. Propagation of error shows that a single four-component calibration with duplicate injections of each unknown is sufficient to determine log Kow with a precision of less than 0.1 log units.