Epidermis Extracts (Chalone) Inhibit Cell Flux At the G1-S, S-G2and G2-M Transitions In Mouse Epidermis

Publisher Website
Google Scholar
Abstract
Epidermal cell flux at the G1-S, S-G2 and G2-M transition was examined during the 1st 4 h after injection of epidermis extract [chalone]. Flux parameters were estimated by a combination of several methods. G1-S and S-G2 transit rates were calculated on the basis of a double labeling technique with [3H]TdR [thymidine-methyl], G2-M flux by colcemid, and relative proportion of cells in the S or G2 phase by flow cytometry. All experiments were performed in early morning and late evening, corresponding to maximum and minimum rates of epidermal cell proliferation in the hairless mouse. Epidermis extract inhibited S-G2 and G2-M transit rates to the same degree, while inhibition of cell flux at the G1-S transit was consistently stronger. Inhibition of cell flux at the different transitions was most pronounced when the rate of cell proliferation was low and vice versa.

This publication has 5 references indexed in Scilit: