GC/MS Analysis of Five Common Benzodiazepine Metabolites in Urine as tert-Butyl-dimethylsilyl Derivatives
- 1 March 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 17 (2) , 114-116
- https://doi.org/10.1093/jat/17.2.114
Abstract
A method for the GC/MS confirmation of oxazepam, nordiazepam, desalkylflurazepam, temazepam, and α-hydroxyalprazolam in urine is described. The method incorporates a solid phase extraction technique developed by Varlan which was found to extract all five metabolites with recoveries exceeding 73%. The extracted metabolites were derivatized with N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide (MTBSTFA), resulting in the formation of stable tert-butyldimethylsilyl (TBDMS) derivatives. Quantitation was performed by GC/MS using oxazepam-D5, nordiazepam-D5, and α-hydroxyalprazolam-D5 as internal standards. The method was found to be linear over the range of 50–2000 ng/mL. Within-run precision, measured as the coefficient of variation at 100 ng/mL, was less than 3% for all analytes except temazepam which was 6% (N = 20).Keywords
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