Determination of Guanine Nucleotide Sensitivity of [125I]-Neuropeptide Y Binding in the Rat Pituitary Gland by Quantitative Autoradiography
- 1 January 1990
- journal article
- research article
- Published by S. Karger AG in Neuroendocrinology
- Vol. 52 (4) , 361-367
- https://doi.org/10.1159/000125608
Abstract
[l25I]-neuropeptide Y receptors were characterized in the rat pituitary gland using quantitative autoradiography. Scatchard analysis of saturation isotherms showed high affinity (Kd 8.5 × 10–11M) and single class of binding sites (Bmax 0.22 pmol/mg of protein) in the posterior lobe of the pituitary gland, with no specific binding in the intermediate or anterior lobe. Increasing concentrations of guanine nucleotide analogues and peptide YY inhibited neuropeptide Y binding. In addition, neuropeptide Y binding was pertussis-toxin-sensitive. These results add additional information about a possible neuronal (through neuropeptide Y) and hormonal (through peptide YY) regulation of posterior pituitary function, which is most probably GTP-dependent.Keywords
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