Pattern of RNA Transcription during Bacillus subtilis Spore Outgrowth

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Abstract
During early outgrowth of B. subtilis spores, there is a period of RNA and protein synthesis in the absence of DNA replication. Two mutants of B. subtilis, PB2442 (gsp-4) and PB2452 (gsp-81), were used to study the pattern of RNA synthesis during this period. The 2 mutants are temperature-sensitive in the outgrowth phase, and show a limited amount of incorporation of [3H]uridine at 47.degree. C. The RNA synthesized during a 2 min pulse with [3H]uridine were hybridized to EcoRI-digested DNA, after agarose gel electrophoresis and transfer to nitrocellulose paper (Southern technique). For both mutants the transcripts synthesized at 35.degree. C at different times were different. Differences were also observed in the transcripts made at 47.degree. C. For both mutants, in the presence of chloramphenicol, the same hybridization pattern was obtained for RNA pulse-labeled at different periods during outgrowth.