Early changes in glucose and phospholipid metabolism following apoptosis induction by IFN‐γ/TNF‐α in HT‐29 cells
- 13 May 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 544 (1-3) , 123-128
- https://doi.org/10.1016/s0014-5793(03)00489-7
Abstract
The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5’-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumorsKeywords
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