Identification of CD8+T Cell Epitopes in the Immediate Early 62 Protein (IE62) of Varicella‐Zoster Virus, and Evaluation of Frequency of CD8+T Cell Response to IE62, by Use of IE62 Peptides after Varicella Vaccination
Open Access
- 1 July 2003
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 188 (1) , 40-52
- https://doi.org/10.1086/375828
Abstract
Varicella-zoster virus (VZV) causes varicella, establishes neuronal latency, and can reactivate, resulting in herpes zoster. VZV-specific T cells are important for controlling infection. VZV immediate early protein 62 (IE62) is recognized by cytotoxic T cells from immune individuals, but no CD8+ T cell epitopes have been defined for any VZV protein. CD8+ T cell frequencies were assessed by cytokine flow cytometry (CFC), by use of synthetic-peptide pools corresponding to the IE62 sequence. IE62 peptide-specific CD8+ T cells were below the threshold of detection, by direct CFC of either whole blood or peripheral blood mononuclear cells (PBMCs). Activated CD8+CD69+ T cells that produced interferon-γ were detectable after in vitro restimulation of PBMCs, and restricted epitopes were identified for HLA-A*0201-positive subjects. Varicella vaccination of 3 VZV-immune subjects was associated with increases in IE62 peptide-specific CD8+ T cells, a finding indicating that in vivo re-exposure boosts memory immunity to this important viral protein.Keywords
This publication has 0 references indexed in Scilit: