Metabotropic responses to acetylcholine and serotonin of Xenopus oocytes injected with rat brain mRNA are transduced by different G‐protein subtypes
- 9 March 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 299 (2) , 179-182
- https://doi.org/10.1016/0014-5793(92)80242-9
Abstract
To assign the GTP‐binding protein (G‐protein) subtype involved in the signal transduction from exogenous receptors to phospholipase C in the Xenopus oocyte translation system, antisense DNA complementary to rat G‐protein α‐subunit MRNA was designed and injected together with rat brain poly(A)+ RNA. Current response of mRNA‐injected oocytes to acetylcholine (ACh) was suppressed dose‐dependently by a co‐injection of Gilα‐antisense DNA, but response of the same oocytes to serotonin (5‐HT) was not inhibited. In the oocytes co‐injected with Goα‐antisense DNA, the 5‐HT response was more effectively suppressed than the ACh response. These results suggest that Goα but not Gilα intermediates brain 5‐HTIC receptor function, and in contrast, muscarinic receptors derived from rat brain utilize Gilα rather than Goα to activate phospholipase C.Keywords
This publication has 13 references indexed in Scilit:
- Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currentsNature, 1991
- Molecular cloning and sequence determination of four different cDNA species coding for α‐subunits of G proteins from Xenopus laevis oocytesFEBS Letters, 1990
- Molecular cloning of a novel splice variant of the alpha subunit of the mammalian Go protein.Journal of Biological Chemistry, 1990
- Goprotein as signal transducer in the pertussis toxin-sensitive phosphatidylinositol pathwayNature, 1990
- Molecular cloning and sequence determination of a cDNA coding for the α‐subunit of a Go‐type protein of Xenopus laevis oocytesFEBS Letters, 1989
- Phorbol Ester Inhibition of Current Responses and Simultaneous Protein Phosphorylation in Xenopus Oocyte Injected with Brain mRNAJournal of Neurochemistry, 1988
- GTP-binding proteins Gi and Go transplanted onto Xenopus oocyte by rat brain messenger RNAMolecular Brain Research, 1987
- Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium.Journal of Biological Chemistry, 1987
- Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNAMolecular Brain Research, 1986
- Cholinergic and catecholaminergic receptors in the Xenopus oocyte membraneThe Journal of Physiology, 1982