FLUORESCENCE QUENCHING OF 10‐METHYLACRJDINIUM CHLORIDE BY NUCLEOTIDES

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Abstract
Abstract— All nucleotides examined (AMP, GMP, TMP and CMP) quench the fluorescence or 10–methylacridinium chloride (10–MEAC). The fluorescence spectrum of 10–MEAC‐nucleotide system is identical with that of 10–MEAC itself, and the fluorescence decay kinetics follow a single‐exponential decay law. The dependence of fluorescence quantum yields and fluorescence lifetimes upon the concentration of nucleotides indicates that the fluorescence of 10–MEAC is greatly quenched in both dynamic and static processes by nucleotides. The quenching constants increase in the order: AMP ≳ GMP > TMP ≳ CMP. The results of 10–MEAC are compared with those of other acridine dyes (proflavine, 9–aminoacridine and acridine orange).