Response of Sheep Lymphocytes to PHA: Quantitation by Nuclear Volume Measurement and Cell Counts

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Abstract
The response of sheep peripheral blood lymphocytes (PBL) to phytohemagglutinin was studied. Assessment of proliferative response was performed by determination of nuclear volumes and cell counts in cultures from 14 sheep and by incorporation of [3H]-thymidine in cultures in 4 additional sheep. PBL of sheep transformed and proliferated with PHA similarly to human peripheral blood lymphocytes with minor differences. Quantitation of the proliferative response by determining the cell count and nuclear volumes provided more information on cell kinetics in culture than the commonly used isotope-labeled thymidine incorporation method.

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