Effect of Chicken Antiserum and Tissue Extracts on the Oocysts, Sporozoites, and Merozoites of Eimeria tenella and E. acervulina

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Abstract
Sporulated oocysts, liberated sporozoites, and second-generation merozoites of Eimeria tenella and sporozoites of E. acervulina were incubated in phosphate-buffered saline and serum from: (1) disease-free chickens, (2) chickens given a single inoculation of either E. tenella or E. acervulina, and (3) chickens hyperimmunized to these patasites. There was no effect on viability of the sporulated oocysts; however, sporozoites and merozoites of E. tenella were immobilized after 2 and 1 hr, respectively, in serum from E. tenella-hyperimmune birds; and sporozoites of E. acervulina were immobilized in serum from E. acervulina-hyperimmune birds. Within the same time limits there was no immobilization of sporozoites and merozoites incubated in PBS extracts of intestinal and cecal mucosae from these same chickens. In vitro immobilization proved to be true neutralization of the parasites, as birds inoculated via the cloaca with immobilized sporozoites and merozoites either failed to develop patent infections or developed infections of extremely low magnitude.

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