The repression of the reverse‐oriented transcription from the adenovirus terminus by NFI in competition with TFIID
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1) , 53-56
- https://doi.org/10.1016/0014-5793(91)80202-e
Abstract
Nuclear factor 1 (NFI) represses the transcription which is promoted by the cloned adenovirus (Ad) type 5 DNA replication origin and is reverse-oriented with respect to the direction of the replication. The mechanism of this repression by NFI was investigated. In the cell-free transcription system, the repression was observed only when NFI was present during the formation of the transcription initiation complex. From the results of DNase I protection experiments, it was indicated that NFI bound to its binding site in the Ad replication origin prevents TFIID from proper binding to the adjacent AT-rich region and consequently represses the transcription.Keywords
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