The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.
- 1 February 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (3) , 699-702
- https://doi.org/10.1073/pnas.82.3.699
Abstract
Glucocorticoid receptor (GCR) protein stimulates transcription from a variety of cellular genes. GCR partially purified from rat liver binds specifically to a site within the 1st intron of the human growth hormone (hGH) gene, .apprxeq. 100 base pairs downstream from the start of hGH transcription. GCR binding is selectively inhibited by methylation of 2 short, symmetrically arranged clusters of guanine residues within this site. A cloned synthetic 24-base-pair deoxyoligonucleotide containing the predicted GCR binding sequence interacts specifically with GCR. The hGH binding site shares sequence homology with a GCR binding site upstream from the human metallothionein II gene and a subset of GCR binding sites from mouse mammary tumor virus. All of these binding sites for this eukaryotic transcriptional regulatory protein show remarkable similarity in overall geometry to the binding sites for several prokaryotic transcriptional regulatory proteins.This publication has 41 references indexed in Scilit:
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