Deglycosylation by trifluoromethanesulphonic acid of endopeptidase-24.11 purified from pig kidney and intestine

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Abstract
Endopeptidase-24.11, an integral microvillar membrane enzyme, exists in differently glycosylated forms when purified from pig kidney and intestine. When these glycoproteins and another form of the kidney enzyme prepared from the Yucatan dwarf strain of piglet were treated under controlled conditions with trifluoromethanesulfonic acid, the proteins were freed of carbohydrate and all had the same apparent subunit MW (77,000) even though the untreated forms varied from MW 89,000 to MW 95,000.