An Attenuated aroA Salmonella typhimurium Vaccine Elicits Humoral and Cellular Immunity to Cloned -Galactosidase in Mice
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 155 (1) , 86-92
- https://doi.org/10.1093/infdis/155.1.86
Abstract
β-Galactosidase (GZ) is an intracellular protein that is frequently used to express cloned antigens as fusion proteins in Escherichia coli. Salmonella typhimurium strain SL3261, an attenuated aroA vaccine strain, was used as a carrier for the plasmid pXY411, which directs the expression of GZ in salmonellae (which do not normally produce this protein). The resulting strain—SL3261(pXY411)—expressed GZ as an intracellular antigen. The plasmid was stable in vitro and in vivo and did not significantly alter the behavior of strain SL3261 in mice. Animals intravenously vaccinated with this construct developed circulating antibodies to GZ, as measured by ELISA, and delayed hypersensitivity to the antigen injected in the footpad. These results indicate that attenuated salmonellae may be expected to elicit both humoral and cellular responses to intracellular cloned antigens.Keywords
This publication has 9 references indexed in Scilit:
- Identification and location of l-glycerate, an unusual acyl substituent in gellan gumCarbohydrate Research, 1986
- In vivo properties of a cloned K88 adherence antigen determinantInfection and Immunity, 1986
- Bacterially expressed antigenic peptide from foot-and-mouth disease virus capsid elicits variable immunologic responses in animals.The Journal of Immunology, 1986
- Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrheasInfection and Immunity, 1984
- Safety and Antigenicity of Typhoid-Shigella sonnei Vaccine (Strain 5076-1C)The Journal of Infectious Diseases, 1984
- AROMATIC-DEPENDENT SALMONELLA-TYPHIMURIUM AS MODIFIED LIVE VACCINES FOR CALVES1984
- Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccinesNature, 1981
- NATURAL-RESISTANCE TO SALMONELLA-TYPHIMURIUM IN DIFFERENT INBRED MOUSE STRAINS1979
- The transposon Tn1 as a probe for studying ColE1 structure and functionMolecular Genetics and Genomics, 1977