Preparation of blood globin through carboxymethyl cellulose chromatography
- 1 February 1981
- journal article
- Published by Oxford University Press (OUP) in International Journal of Food Science & Technology
- Vol. 16 (1) , 81-91
- https://doi.org/10.1111/j.1365-2621.1981.tb00999.x
Abstract
Summary: A simple and inexpensive procedure for globin preparation from blood of various animals was developed in order to use globin as human food. Carboxymethyl cellulose was most effective among some cation exchangers tested, although amounts of haemoglobin applied to 1 g of the exchanger should be limited to less than 70 mg. The behaviour of adsorbing haem or haemin to the exchanger could not be explained only by a general theory of ion exchange chromatography. Yields and Fe contents of globin preparations from various haemoglobins by this procedure ranged from 67 to 80% and from 0.008 to 0.03% respectively, except for a chicken haemoglobin sample.Keywords
This publication has 6 references indexed in Scilit:
- Protein concentrates from slaughter animal bloodInternational Journal of Food Science & Technology, 1977
- FUNCTIONAL PROPERTIES OF PROTEINS ISOLATED FROM BOVINE BLOOD BY A CONTINUOUS PILOT PROCESSJournal of Food Science, 1975
- A spectroscopic study of haematin compounds in the Soret regionBiochimica et Biophysica Acta, 1963
- The Iron–Protein Link in Hæmoglobin and MyoglobinNature, 1959
- Studies on the structure of hemoglobin I. Physicochemical properties of human globinBiochimica et Biophysica Acta, 1958
- Heme ProteinsAdvances in Protein Chemistry, 1948