Incorporation of Fatty Acids into Phosphatidylcholine by Acyl-CoA: l-acyl-sn-glycero-3-phosphorylcholine Acyltransferase in Liver of Rainbow trout, Salmo gairdneri

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Abstract
The formation of phosphatidylcholine by acylation of 1-acyl-sn-glycero-3-phosphorylcholine with fatty acids was demonstrated in microsomal preparations from the livers of rainbow trout (Salmo gairdneri). Maximum incorporation of fatty acids into phosphatidylcholine was highly dependent upon the addition of 1-acyl-sn-glycero-3-phosphorylcholine to the incubation medium when activated acids in the form of [14C]acyl-CoA esters were provided. The acyl-CoA: 1-acyl-sn-glycero-3-phosphorylcholine acyltransferase showed a selectivity for unsaturated (oleate) over saturated (palmitate) fatty acids when microsomes were prepared from rainbow trout acclimated to 7 or 15 C and assays conducted at both temperatures. The presence of an acyl-CoA hydrolase in the microsomal preparations is also reported. The possible function of the acyltransferase in rearranging the fatty acid composition of cellular phospholipid when fish are exposed to a change in environmental temperature is discussed.

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