Correlation of Cellular Aggregation of Transformed Cells with Their Growth in Soft Agar and Tumorigenic Potential

Abstract
Formation of cellular aggregates by rat, mouse, hamster and human transformed cells was compared to aggregate formation by untransformed control cells. Viability and proliferation of cells in the aggregate form correlated well with tumorigenic potential regardless of the method of transformation (spontaneous, chemical or virus-induced). This assay has the potential for a fast and accurate means of evaluating in vitro transformation.

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