Role of intracellular loops of cannabinoid CB1 receptor in functional interaction with Gα16
- 9 June 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 522 (1-3) , 130-134
- https://doi.org/10.1016/s0014-5793(02)02917-4
Abstract
The cannabinoid CB1 but not the CB2 receptor was demonstrated to couple via Gα16 to activate phospholipase C after co-expression in COS7 cells. Chimeric CB1/CB2 receptors were used as a model to study receptor–Gα16 interaction. Sequences of the second and third intracellular loops and the carboxy-terminus were substituted from the CB1 into the CB2 receptor. Only the triple mutant with all three regions replaced activated phospholipase C to a similar extent as the CB1 receptor, suggesting that all three intracellular regions are required for interacting with Gα16. Several sub-domains within the third intracellular loop were identified for receptor–Gα16 interactionKeywords
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