Location of Tetrachlorsalicylanilide Taken up by Bacillus megaterium

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Abstract
C14-Tetrachlorsalicylanilide (TCS) was taken up by Bacillus megaterium, became localized on the cyto-plasmic membrane and was recovered from the membrane by treatment with normal horse serum. Normal phase-dark B megaterium protoplasts became phase-bright and distorted on addition of TCS; osmotically resistant "protoplasts" in buffer were obtained from TCS-treated B megaterium subsequently treated with lysozyme. Both these conditions were inhibited or annulled by serum.